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cd15 fitc  (Miltenyi Biotec)


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    Miltenyi Biotec cd15 fitc
    Cd15 Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd15 fitc/product/Miltenyi Biotec
    Average 94 stars, based on 7 article reviews
    cd15 fitc - by Bioz Stars, 2026-02
    94/100 stars

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    Plots illustrating the gating strategy for neutrophil subpopulations. The initial gate was set on FSC-A and FSC-H to exclude doublets, followed by gating on CD45 high to eliminate debris, and FSC-H and SSC to exclude degranulated cells. Neutrophils were identified as CD45 high CD14 low <t>CD15</t> high CD193 low cells and further classified into subpopulations based on surface expression of CD62L and CD16: CD16 high CD62L high mature neutrophils (MN), CD16 low CD62L high banded neutrophils (BN), CD16 high CD62L low hypersegmented neutrophils, and CD16 low CD62L low neutrophils. Representative data from one healthy donor (HD), one Non-SIRS patient, and one SIRS patient were analyzed using the Kaluza software.
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    Plots illustrating the gating strategy for neutrophil subpopulations. The initial gate was set on FSC-A and FSC-H to exclude doublets, followed by gating on CD45 high to eliminate debris, and FSC-H and SSC to exclude degranulated cells. Neutrophils were identified as CD45 high CD14 low <t>CD15</t> high CD193 low cells and further classified into subpopulations based on surface expression of CD62L and CD16: CD16 high CD62L high mature neutrophils (MN), CD16 low CD62L high banded neutrophils (BN), CD16 high CD62L low hypersegmented neutrophils, and CD16 low CD62L low neutrophils. Representative data from one healthy donor (HD), one Non-SIRS patient, and one SIRS patient were analyzed using the Kaluza software.
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    Plots illustrating the gating strategy for neutrophil subpopulations. The initial gate was set on FSC-A and FSC-H to exclude doublets, followed by gating on CD45 high to eliminate debris, and FSC-H and SSC to exclude degranulated cells. Neutrophils were identified as CD45 high CD14 low CD15 high CD193 low cells and further classified into subpopulations based on surface expression of CD62L and CD16: CD16 high CD62L high mature neutrophils (MN), CD16 low CD62L high banded neutrophils (BN), CD16 high CD62L low hypersegmented neutrophils, and CD16 low CD62L low neutrophils. Representative data from one healthy donor (HD), one Non-SIRS patient, and one SIRS patient were analyzed using the Kaluza software.

    Journal: Frontiers in Immunology

    Article Title: Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populations

    doi: 10.3389/fimmu.2025.1565606

    Figure Lengend Snippet: Plots illustrating the gating strategy for neutrophil subpopulations. The initial gate was set on FSC-A and FSC-H to exclude doublets, followed by gating on CD45 high to eliminate debris, and FSC-H and SSC to exclude degranulated cells. Neutrophils were identified as CD45 high CD14 low CD15 high CD193 low cells and further classified into subpopulations based on surface expression of CD62L and CD16: CD16 high CD62L high mature neutrophils (MN), CD16 low CD62L high banded neutrophils (BN), CD16 high CD62L low hypersegmented neutrophils, and CD16 low CD62L low neutrophils. Representative data from one healthy donor (HD), one Non-SIRS patient, and one SIRS patient were analyzed using the Kaluza software.

    Article Snippet: Neutrophils were characterized using a combination of fluorescently labeled human monoclonal antibodies: CD15 FITC (clone MEM-158), CD193 PE (clone 5E8), CD45 PE-DY647 (clone MEM-28), CD14 PerCP Cy5.5 (clone MEM-15), CD62L PC7 (clone LT-TD180), CD10 APC (clone MEM-78), CD11b PB (clone ICRF44), and CD16 PO (clone 3G8) (all antibodies from Exbio Praha, Vestec, Czech Republic).

    Techniques: Expressing, Software

    Neutrophil Functional Test: Ingestion capacity in neutrophil subpopulations of FITC-labeled E. coli . Normal ingestion capacity of FITC-labeled E. coli in Non-SIRS and SIRS patients at TP1. (A) CD16 low CD62L low neutrophils exhibit reduced ingestion capacity of E. coli (B, C) compared to mature neutrophils (MN). The functional properties of both populations remained unchanged over time (TP1 and TP2) (D) . The data are presented as the mean fluorescence intensity of FITC-labeled E. coli . Data were non-normally distributed (Shapiro-Wilk test), and statistical analysis was conducted using the Kruskal-Wallis test with Dunn’s correction for multiple comparisons (all vs. all); ****p ≤ 0.0001. Sample sizes: Ingestion of E. coli (MFI E. coli FITC): TP1, TP2: N = 35 healthy controls, 12 Non-SIRS patients, 6 SIRS patients.

    Journal: Frontiers in Immunology

    Article Title: Pro-inflammatory role of neutrophils populations in trauma patients: monitoring neutrophil populations

    doi: 10.3389/fimmu.2025.1565606

    Figure Lengend Snippet: Neutrophil Functional Test: Ingestion capacity in neutrophil subpopulations of FITC-labeled E. coli . Normal ingestion capacity of FITC-labeled E. coli in Non-SIRS and SIRS patients at TP1. (A) CD16 low CD62L low neutrophils exhibit reduced ingestion capacity of E. coli (B, C) compared to mature neutrophils (MN). The functional properties of both populations remained unchanged over time (TP1 and TP2) (D) . The data are presented as the mean fluorescence intensity of FITC-labeled E. coli . Data were non-normally distributed (Shapiro-Wilk test), and statistical analysis was conducted using the Kruskal-Wallis test with Dunn’s correction for multiple comparisons (all vs. all); ****p ≤ 0.0001. Sample sizes: Ingestion of E. coli (MFI E. coli FITC): TP1, TP2: N = 35 healthy controls, 12 Non-SIRS patients, 6 SIRS patients.

    Article Snippet: Neutrophils were characterized using a combination of fluorescently labeled human monoclonal antibodies: CD15 FITC (clone MEM-158), CD193 PE (clone 5E8), CD45 PE-DY647 (clone MEM-28), CD14 PerCP Cy5.5 (clone MEM-15), CD62L PC7 (clone LT-TD180), CD10 APC (clone MEM-78), CD11b PB (clone ICRF44), and CD16 PO (clone 3G8) (all antibodies from Exbio Praha, Vestec, Czech Republic).

    Techniques: Functional Assay, Labeling, Fluorescence